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Subcellular distribution and dynamics of Gal80. Sc862 (Gal80-2GFP) cells expressing Htb2-mCherry from pFJ35 were grown to mid-log phase in glycerol–lactic acid media. The cells were induced with galactose for 4–6 hr to enhance the GFP signal and then shifted back to non-inducing media for 4 hr. The (A) nuclear or (B) cytoplasmic region was briefly bleached.
