Figure 2—

pMad-Gsk is expressed in larval wing SOP and responds to Wg signal transducers. Stacked confocal images of the presumptive wing blade from larvae aged 116–120 hr AEL with anterior to the left (as in Figure 1A, left inset) displaying pMad-Gsk (green), Ac (nuclear; red), and Sens (nuclear; blue) except as noted. We maintain this color scheme in Figures 2, 4, 5, 6, and 7. (A) Sca-Mad disk appears wild type with Ac and Sens in two rows of SOP (middle arrowhead; Ac anterior margin only). Ac and Sens expressing SOP for sensilla (L1: left arrowhead; L3: right arrowhead) are also visible. (B) Sca-Mad disk with several SOP on the dorsal margin and L1/L3 regions that express nuclear Ac and Sens and uniform (cytoplasmic and nuclear) pMad-Gsk. Boxed area is magnified twofold and shown as two insets to document the subcellular localization of pMad-Gsk: three-color (left) and two-color (pMad-Gsk and Ac; right). The ventral-most of the three pMad-Gsk cells to the right of the boxed area is a rare example that does not also express either Ac or Sens. (C) Sca-Mad disk labeled with Wg instead of Ac. Several cells on the margin and L1/L3 sensilla regions that express nuclear Sens also express cytoplasmic pMad-Gsk. Overall, pMad-Gsk was seen in 10.2 ± 6.7 cells (n = 108), 4.8 ± 4.5 anterior margin cells, 2.8 ± 2.8 L1 sensilla region cells, and 2.7 ± 2.1 L3 sensilla region cells. Insets: three-color (left), two-color [pMad-Gsk and Sens (middle)], and pMad-Gsk (right). (D) Sca-Mad,MadRNAi disk with no pMad-Gsk. (E) Sca-MGM disk with no pMad-Gsk, a phenotype also seen with Sca-Mad, MGM. (F) Sca-Mad,CA-Zw3 disk with expanded pMad-Gsk in the L1/L3 sensilla regions and ectopic pMad-Gsk on the ventral surface (above the margin rows). (G) Sca-Mad,Dsh disk displays expanded pMad-Gsk in the L1/L3 sensilla regions. (H) Sca-Mad,DN-Zw3 disk with no pMad-Gsk. (I) Sca-Mad,dAxinΔRGS disk similar to Sca-Mad (no effect on pMad-Gsk). (J) ScaMad,DN-Tkv disk similar to Sca-Mad (no effect on pMad-Gsk).