Figure 5 .

Cardiac-specific knockdown of ribosomal proteins in the adult results in postdevelopmental dilated cardiac phenotype. (A) Representative OCT images of Drosophila containing the following transgenes: tub-Gal80^ts, tinC-Gal4 drivers control; tub-Gal80^ts, tinC-Gal4, and UAS-RNAi for RpS15Aa; tinC-Gal4 and UAS-RNAi for RpS3; tub-Gal80^ts, tinC-Gal4, and UAS-RNAi for RpS5a; tub-Gal80^ts; tinC-Gal4 and UAS-RNAi for mRpS33. Flies developed at 18° keeping the RNAi transgene from being expressed. Adult flies were either kept at 18° (no knockdown) or moved to 27° to turn on heart-specific knockdown in the adult. (B) Summary data showing that with drivers only, heart function remains normal when adults are moved to 27°; however, heart function is significantly impaired when RNAi expression is induced in the adult heart. n = 9–17 flies per group shown in parentheses. *P < 0.05 vs. 18° control, unpaired t-test. No error bars are shown on mRpS33 at 18° since all measurements of FS were 100%.