Figure 4.

Detection of photosynthetic and protein assembly-specific proteins in chromoplasts and chloroplasts. Isolated pea chloroplasts (lanes CHL) and red pepper chromoplasts containing 3 μg chlorophyll/mg protein (lanes CHR) representing total plastid (lanes T), soluble (stromal) plastid (lanes S), and membrane plastid (lanes M) fractions were subjected to immunoblot analysis (see Methods). Immunoblots were probed with antibodies against: Rbcs (A), OE23 (B), LHCP (C), Hsp70 (D), cpSecA (E), Toc75 (F), Pftf (G), and cpSRP54 (H) and visualized by enhanced chemiluminescence.