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. 1999 Feb;119(2):575–584. doi: 10.1104/pp.119.2.575

Figure 5.

Figure 5

Import and subplastid localization of precursors. Isolated chromoplasts containing 2.0 μg chlorophyll/mg protein were incubated with radiolabeled in vitro translation products (lanes tp) in the presence (+ATP) or absence (−ATP) of ATP, as described in Methods. Following import, chromoplasts were recovered without protease posttreatment (lanes C) or with protease posttreatment (lanes CP). Untreated chromoplasts were subfractionated into stroma (lanes S) and total membranes (lanes TM). Equivalent aliquots of membranes were extracted with NaOH (lanes MN) or treated with protease (lanes MP). One microliter of radiolabeled translation product and 15 μL of each sample (representing 7.5 μL of the original radiolabeled translation product added to the import reaction) were analyzed by SDS-PAGE (A–D, 12.5%; E, 7.5%) and fluorography. Precursors utilized for import were: Rbcs (A), OE17 (B), OE33 (C), LHCP (D), and Pftf (E). p, pOE17; i, iOE17; m, mOE17.