Figure 5. Expression of MuRF1 and MAFbx in wild type (WT) and MuRF1 null (KO) mice after dexamethasone (DEX) treatment.
MuRF1 and MAFbx expression was measured in the triceps surae of female mice after either 3 or 14 days of DEX treatment. A, quantitative PCR was performed to measure MuRF1 expression in control (filled bars) and DEX-treated (open bars) WT and MuRF1 KO mice. Expression values were normalized to Rpl39 and expressed as mean ± SD (n = 3–4 per group). As expected, MuRF1 expression was not detected in the KO mice. *P < 0.05. B, MAFbx expression was assessed by Northern blot and quantified by densitometry in control (filled bars) and DEX-treated (open bars) WT and MuRF1 KO mice. *P < 0.01.