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Temporal expression of Gtr mRNA in developing nodules and comparison with Enod2 and Lb. Approximately 5 μg of poly(A+) RNA from uninfected roots (U) and from nodules 10, 13, 16, 19, and 25 d postinfection were loaded onto each lane. A single filter was hybridized with each radiolabeled cDNA separately, and the filter was stripped after each hybridization and exposure.
