FIG. 5.

Expression of Plk3 correlates with the cell cycle progression in CD4⁺ T cells. Lysates from the HSRM1 cell line were assayed for the expression of Plk3, Cdc25C, and cyclin B at various stages of the cell cycle. Cells were either nonsynchronized (ctrl), synchronized at G₁/S phase with aphidicolin (G1/S) or at G₂/M phase with nocodazole (M), or assayed 2 h after the release of the nocodazole block (M+2). Cdc25C analysis shows the nonphosphorylated-inactive form (c), the single-phosphorylated-inactive form (b), and the double-phosphorylated-active form (a). β-Actin expression was used as a control for the equivalent loading.