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. Author manuscript; available in PMC: 2011 Nov 11.
Published in final edited form as: Biochem J. 2011 Jul 1;437(1):43–52. doi: 10.1042/BJ20110410

Figure 1. ATPase activity of FlaI under various conditions.

Figure 1

Total Pi released upon ATP (NTP) hydrolysis was measured using the Malachite Green assay. (A) Influence of pH on FlaI ATPase activity. (B) Influence of temperature on FlaI ATPase activity. (C) Preference for optimal divalent metal ion as a cofactor in FlaI ATPase activity. The final concentration of protein used in the assays was 1 μM and the final concentration of metal ions was 5mM. (D) Analysis of optimal substrate for hydrolysis. NTPs were added to a final concentration of 5 mM. (E) FlaI ATPase activity as a function of the ATP concentration measured at 70°C with 1 μM FlaI. (F) The same FlaI ATPase activity plotted according to the Hill equation (Hill equation = 2.0). All experiments were performed in triplicate and S.D. values are indicated.