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. Author manuscript; available in PMC: 2011 Nov 11.
Published in final edited form as: Biochem J. 2011 Jul 1;437(1):43–52. doi: 10.1042/BJ20110410

Figure 2. Effect of tetraether lipids from S. acidocaldarius and total lipids from E. coli on FlaI ATPase activity.

Figure 2

ATP hydrolysis was measured at 70°C with 1 μM purified FlaI in the presence of different concentrations (40–800 μg) of isolated tetraether-linked lipids from S. acidocaldarius, and total lipids from E. coli against respective controls in which an equivalent amount of respective lipids were present in the assay mixture without any protein, and treated in a similar manner as for the enzymatic reaction described in the Materials and methods section. All experiments were performed in triplicate and S.D. values are indicated.