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. Author manuscript; available in PMC: 2012 Nov 15.
Published in final edited form as: Dev Biol. 2011 Sep 7;359(2):242–250. doi: 10.1016/j.ydbio.2011.08.026

Figure 1. Blockage of the third ventricle is a feature of HPE in Shh null embryos and is rescued by removing one functional copy of Gli3.

Figure 1

The telencephalic vesicle (tel) in E10.5 embryos was injected with a solution of fast green to fill the ventricular system, with a hole cut in the mesencephalon to allow the dye to exit. The lateral ventricles and third ventricle are filled with dye in a wildtype embryo (A). Ventricular flow is blocked at the level of the third ventricle in a Shh null embryo, so that the forebrain ventricular system forms a sealed monoventricle (B). In contrast, ventricular system continuity is restored in a Shh−/−;Gli3+/XtJ embryo (C), although the diencephalic ventricle (di) is reduced in size compared with wildtype. Scale bar is 700µm.