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. 2004 Feb;78(3):1411–1420. doi: 10.1128/JVI.78.3.1411-1420.2004

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FIG. 5. — Detergent sensitivity of mutant particles. Particles were collected from culture fluids, treated with 1% NP-40, centrifuged, and analyzed by immunoblotting with anti-CA antiserum as described in Materials and Methods. (A) Lanes: 1 and 2, wild type; 3 and 4, PR⁻; 5 and 6, S1D; 7 and 8, S2D. Lanes 1, 3, 5, and 7, pellets; lanes 2, 4, 6, and 8, supernatants. (B) Lanes: 1 and 2, wild type; 3 and 4, PR⁻; 5 and 6, S3R. Lanes 1, 3, and 5, pellets; lanes 2, 4, and 6, supernatants. In control samples not exposed to NP-40, all of the CA-containing proteins were found exclusively in the pellet fraction in all samples (data not shown).