FIG. 10.

Reaction conditions for the formation of the covalent m⁷GMP-enzyme intermediate when m⁷GTP was used as the substrate. The reactions, catalyzed by the wild-type enzyme, were carried out at 30°C for 80 min under conditions similar to those for the standard assay for the formation of the m⁷GMP-enzyme intermediate, except that [α-³²P]GTP was replaced by [α-³²P]m⁷GTP, and AdoMet, AdoHcy, GTP, and m⁷GTP (each 100 μM) were included in the reaction mixture at different combinations as indicated.