Fig. 5.

3D neuron patterning on the NMM. Fibers of NMM compose 3D grid pattern which is a guide-way to the neurites and these 3D structural patterns of NMM appropriate to neuronal patterning. (A) Cacein AM labeled live neurons. Arrows indicate that neurites growth along with 3D grid pattern. (B) SEM image of patterned neuron. (C) Confocal laser scanning microscope image also show the patterned neurons on the NMM. All of neurons were extracted in hippocampus and stained at 6DIV. Diameter of pore is 20 µm.