FIG. 1.

Schematic representation of Gag fusion proteins and Gag-Gag FRET. (A) Diagram of all Gag fusion expression constructs. All six constructs were made as CFP and YFP fusions. (B) Gag-Gag FRET is represented, with the Gag C terminus fused to CFP or YFP with a flexible linker (left). The excitation and emission wavelengths for CFP and YFP are shown to the right. Where two wavelengths are listed, the upper wavelength represents the primary excitation-emission peak and the lower represents the secondary peak.