FIG. 3.

Maturation of envelope-specific antibody avidity in monkeys infected with glycosylation mutant viruses. Serum antibody responses from monkeys infected with SIVmac239 (WT) or glycosylation mutant viruses g45, g46, g56, or M5 were analyzed for reactivity to SIVsmB7 envelope proteins in the ConA ELISA. Serum antibody responses from monkeys infected with each glycosylation mutant virus are shown as open symbols and compared to the average response from monkeys infected with WT virus (black squares). Antibody avidity was determined by measuring the stability of the antigen-antibody complexes to 8 M urea and is expressed as the (OD of wells washed with 8 M urea/OD of wells washed with PBS) × 100.