FIG. 4.

Maturation of envelope-specific antibody conformational dependence in monkeys infected with glycosylation mutant viruses. Serum antibody responses from monkeys infected with SIVmac239 (WT) or glycosylation mutant viruses g45, g46, g56, or M5 were analyzed for reactivity to SIVsmB7 envelope proteins in the ConA ELISA. Serum antibody responses from monkeys infected with each glycosylation mutant virus are shown as open symbols and compared to the average response from the monkeys infected with WT virus (black squares). Conformational dependence was determined by measuring the reactivity to native versus denatured envelope proteins; ratios of >1 reflect predominant reactivity with native envelope proteins, while ratios of <1 reflect predominant reactivity with denatured envelope proteins.