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. 2004 Feb;78(3):1440–1447. doi: 10.1128/JVI.78.3.1440-1447.2004

FIG. 1.

FIG. 1.

(A) Schematic representation of the CMV genome with the UL111.5A transcript expanded to show the position of primers (arrow heads) and probe (open box) used in the present study. (B) RT-PCR analysis of UL111.5A region gene expression in latently infected GM-Ps. Detection of UL111.5A region transcripts in CMV stain Toledo productively infected HFFs (day 4 postinfection) and latently infected GM-Ps (day 14 postinfection) (B) and CMV strain Towne (C) and AD169 (D) latently infected GM-Ps (day 14 postinfection). Panels B to D show ethidium bromide-stained agarose gels (upper panel) and the corresponding Southern blots (lower panel) of products generated from RT-PCR analysis for UL111.5A region transcripts. The sizes of the RT-PCR products are indicated with arrows and the numbers to the left of the gels indicate the size of the adjacent molecular weight size markers (M). The presence (+) or absence (−) of reverse transcriptase (RT) in the reaction mixture is indicated.