FIG. 2.

(A) Enumeration of GM-Ps expressing UL111.5A region transcripts. Southern blot of RT-PCR products showing detection of UL111.5A region transcripts. Latently infected GM-Ps were counted and serially diluted to give 39, 19, 9, 4, 2, and 1 cell per reaction as indicated above lanes. Arrows adjacent to the lanes indicate the position of predicted spliced (512 bp) and unspliced (588 bp) RT-PCR products amplified with the primers JAS-F1 and JAS-R5. GM-Ps from a mock-infected (Mock) or latently infected (Inf GM-P) culture, a sample without RNA (No RNA), and the products of a PCR for UL111.5A region DNA were included as controls. The presence (+) or absence (−) of RT in each reaction is indicated. An ethidium bromide-stained 100-bp DNA ladder (M) is shown on both sides of the panel. (B) Ethidium bromide-stained gel showing RT-PCR detection of GAPDH transcripts (296-bp product) in the same RNA samples as panel A.