FIG. 3.

(A) Determination of 3′ terminus of UL111.5A region transcripts expressed during latent CMV infection of GM-Ps. Ethidium bromide-stained agarose gel of 3′ RACE PCR products derived from RNA extracted from GM-Ps latently infected with CMV strain Toledo. The arrow indicates a single 3′ RACE PCR product of approximately 600 bp after nested amplification with the primers JAS-F1 and UPM for the first round and the primers JAS-P3 and NUP for the second round. A negative control containing no RNA template and a 100-bp DNA ladder (M) are indicated. (B and C) Determination of the 5′ terminus of UL111.5A region transcripts expressed during latent CMV infection of GM-Ps. Southern blots of RT-PCR products were derived from RNA extracted from mock- or CMV strain Toledo-infected GM-Ps. (B) RT-PCR products amplified with the forward primer JAS-52. Arrows indicate the position of 579-bp spliced and 655-bp unspliced products. (C) RT-PCR products amplified with the forward primer JAS-53. Arrows indicate the position of the predicted 712-bp unspliced product, but no spliced product was detected. DNA extracted from productively infected HFFs was included as a positive control for the PCRs. A negative control containing no RNA template and a 100-bp DNA ladder (M) are indicated. The presence (+) or absence (−) of RT in each reaction mixture is indicated.