FIG. 4.

(A) Summary of the structure of UL111.5A region transcripts expressed during productive and latent infection. Upper lines show the double-spliced UL111.5A transcript expressed during productive infection and the corresponding 175-amino-acid ORF that encodes cmvIL-10. Lower lines show the single spliced UL111.5A region transcript expressed during latent infection and its corresponding ORF, which encodes a putative 139-amino-acid protein termed LA-cmvIL-10. Black boxes and open boxes depict transcripts and ORFs, respectively. Nucleotide position numbers (AD169 genome) of the start and stop sites of transcription are indicated, and the region encompassing the start site of the spliced transcript expressed during latency is shown as a gray box. The first methionine residue is indicated by a right-angled arrow. (B) Alignment of the amino acid sequences of human IL-10 and LA-cmvIL-10. Identical amino acids are shown in white letters on a black background and conserved amino acids are shown as “+” signs. Amino acid residues are numbered from the first methionine. The amino acid sequence of the IL-10 homolog expressed by CMV during productive infection (cmvIL-10) is also shown.