FIG. 5.

(A and C) Spliced UL111.5A-region transcripts are expressed during natural latent infection. A Southern blot of MPB (MPB1 to MPB4) and BM (BM1 to BM5) samples after heminested PCR amplification with the primers JAS-F1 and JAS-B1 (first round) and with the primers JAS-F1 and JAS-R1 (second round) was carried out. The arrow indicates the position of a predicted 171-bp spliced UL111.5A region transcript product. A negative control containing no RNA template and a 100-bp DNA ladder (M) are indicated. The presence (+) or absence (−) of RT in each reaction mixture is indicated. The CMV serostatus of each donor is indicated as positive (pos), negative (neg), or not determined (ND). (B and D) Detection of GAPDH by RT-PCR in the same RNA samples as in panels A and C. The arrow indicates the position of a predicted 296-bp GAPDH transcript product.