TABLE 1.
Primer | Sequence | Pair | Predicted product size (bp)a
|
||
---|---|---|---|---|---|
Unspliced | Spliced intron 1 | Spliced introns 1 and 2 | |||
JAS-53 | 5′-ACTATTCTAACCGCGGAAG-3′ | JAS-R4 | 712 | 636 | 553 |
JAS-R5 | 803 | 727 | 644 | ||
JAS-52 | 5′-CATAAAGGACCACCTACCTGGGA-3′ | JAS-R4 | 655 | 579 | 496 |
JAS-R5 | 746 | 670 | 587 | ||
JAS-F1 | 5′-TACAAAGCCGCAGTGTCGTCCAGAGGATTACG-3′ | JAS-R1 | 247 | 171 | N/A |
JAS-B1 | 346 | 270 | N/A | ||
JAS-R5 | 588 | 512 | 429 | ||
JAS-P3 | 5′-CAGATTGCAAGATCTCCGCGTCACCTT-3′ | JAS-R4 | 461 | 385 | 302 |
JAS-R1 | 5′-CAACAACCAGTCCATGACGCTGCATC-3′ | JAS-F1 | 247 | 171 | N/A |
JAS-B1 | 5′-GTAGATGGATTCTAGCGTCGAGCGCAT-3′ | JAS-F1 | 346 | 270 | N/A |
JAS-R4 | 5′-TCCTGAGACAGCCGACTAATCACGGAC-3′ | JAS-53 | 712 | 636 | 553 |
JAS-52 | 655 | 579 | 496 | ||
JAS-P3 | 461 | 385 | 302 | ||
JAS-R5 | 5′-TCTCGAGTGCAGATACTCTTCGAGACGG-3′ | JAS-53 | 803 | 727 | 644 |
JAS-52 | 746 | 670 | 587 | ||
JAS-F1 | 588 | 512 | 429 | ||
JAS-R6 | 5′-GACCACCGTACCGTCGAGCCACACGGAG-3′ | Probe | N/A | N/A | N/A |
NA, not applicable.