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. 2004 Feb;78(3):1440–1447. doi: 10.1128/JVI.78.3.1440-1447.2004

TABLE 1.

Primers and predicted products for PCR analyses

Primer Sequence Pair Predicted product size (bp)a
Unspliced Spliced intron 1 Spliced introns 1 and 2
JAS-53 5′-ACTATTCTAACCGCGGAAG-3′ JAS-R4 712 636 553
JAS-R5 803 727 644
JAS-52 5′-CATAAAGGACCACCTACCTGGGA-3′ JAS-R4 655 579 496
JAS-R5 746 670 587
JAS-F1 5′-TACAAAGCCGCAGTGTCGTCCAGAGGATTACG-3′ JAS-R1 247 171 N/A
JAS-B1 346 270 N/A
JAS-R5 588 512 429
JAS-P3 5′-CAGATTGCAAGATCTCCGCGTCACCTT-3′ JAS-R4 461 385 302
JAS-R1 5′-CAACAACCAGTCCATGACGCTGCATC-3′ JAS-F1 247 171 N/A
JAS-B1 5′-GTAGATGGATTCTAGCGTCGAGCGCAT-3′ JAS-F1 346 270 N/A
JAS-R4 5′-TCCTGAGACAGCCGACTAATCACGGAC-3′ JAS-53 712 636 553
JAS-52 655 579 496
JAS-P3 461 385 302
JAS-R5 5′-TCTCGAGTGCAGATACTCTTCGAGACGG-3′ JAS-53 803 727 644
JAS-52 746 670 587
JAS-F1 588 512 429
JAS-R6 5′-GACCACCGTACCGTCGAGCCACACGGAG-3′ Probe N/A N/A N/A
a

NA, not applicable.