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. 2004 Feb;78(3):1219–1229. doi: 10.1128/JVI.78.3.1219-1229.2004

FIG. 2.

FIG. 2.

Influence of promoters and vector copy numbers on EGFP transgene expression in HOS cells. Cells were transduced with NL-EGFP/CMV, NL-EGFP/CEF, and NL-EGFP/CAG vector stocks at various MOIs in DMEM-10% FBS containing 8 μg of Polybrene/ml at 37°C for 20 h. (A) Percentages of EGFP-positive cells as a function of the amount (nanograms) of p24 added. (B) Percentages of EGFP-positive cells as a function of the number of EGFP transgene copies. (C) MFI values of the EGFP-positive cell populations as a function of the number of EGFP transgene copies. The cells were analyzed by FACS 3 days after transduction. Aliquots were processed for quantitative real-time PCR with EGFP-specific primers. The MFI values and the numbers of EGFP copies per genome are displayed. The data shown were obtained from two independent experiments. Virus titers were 3.6 × 104 infectious units (IU)/ng of p24 for NL-EGFP/CMV, 1.42 × 104 IU/ng of p24 for NL-EGFP/CEF, and 7.6 × 103 IU/ng of p24 for NL-EGFP/CAG.