TABLE 4.
Transduction of MSC progenitor cellsa
| Vector (MOI) | Env GP | Average no. of CFU-Fs per plateb | Result of FACS analysis
|
|
|---|---|---|---|---|
| % Positive cells | MFI value | |||
| Nonec | 21.2 | 0.35 | 50 | |
| Mockd | 17.0 | 0.82 | 113 | |
| NL-EGFP/CMV (0.25) | RD114 | 16.2 | 2.1 | 1,312 |
| NL-EGFP/CMV (0.5) | RD114 | 16.5 | 11.3 | 2,886 |
| NL-EGFP/CMV (1.0) | RD114 | 15.8 | 17.5 | 4,017 |
| NL-EGFP/CMV (3.0) | RD114 | 18.0 | 50.9 | 5,789 |
| NL-EGFP/CMV (2.5) | RD114A | 17.5 | 74.0 | 4,831 |
| NL-EGFP/CMV (5.0) | RD114A | 15.0 | 61.0 | 4,428 |
| NL-EGFP/CMV (10) | RD114A | 15.0 | 50.5 | 5,045 |
| NL-EGFP/CMV (60) | RD114A | 14.0 | 93.4 | 6,785 |
| NL-EGFP/CMV (0.25) | VSV-G | 17.2 | 28.3 | 4,749 |
| NL-EGFP/CMV (0.5) | VSV-G | 14.2 | 36.9 | 3,377 |
| NL-EGFP/CMV (3.0) | VSV-G | 8.5 | 21.7 | 2,801 |
a
For each experiment, 5 × 104 1-day-old cells were transduced in 60-mm-diameter plates at the indicated MOI. MOIs were adjusted based on titers determined on MSCs. The medium was changed 20 h after virus addition, and the cells were analyzed 14 days later. The total number of colonies was determined following staining with crystal violet. The cells from parallel plates were collected and analyzed by FACS.
b
Four plates were used.
c
Cells were grown with medium changes at weekly intervals.
d
Cells were grown in transduction medium in the absence of virus.