Figure 2.
Comparison of SR-NIVI images and H&E-stained histology images. Top row, adipose tissue; middle row, normal mammary tissue; bottom row, tumor tissue. All images are 300 × 300 μm2. The stained sections are 10-μm thick. The SR-NIVI images are 63 × 63 pixels, acquired in less than 5 minutes from 30- to 100-μm thick sections. The bottom 2 SR-NIVI images are full spectral reconstructions with color code (blue >99% probability of normal tissue, red >99% probability of tumor tissue), so tumor incursions less than 100 μm in size can be differentiated readily (see also Fig. 4 and the text). The adipose tissue is a spectral intensity-only NIVI image with color code: white = zero signal, blue = maximum lipid signal.