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The presence of lipid targets does not alter the receptor and pH requirements for conformational change in ALV-A TM. RCASBP(A)-EGFP virus was incubated with increasing concentrations of sTva in the presence or absence of liposomes at pH 5.0 or 7.4 and analyzed for the presence of the 90- and >170-kDa isoforms by TMCA as described in the legend to Fig. 4.
