FIG. 6.

Effects of CMV in endothelial cells. (A) CMV induces ISG expression in endothelial cells. Human fibroblasts or endothelial cells were mock infected (M) or infected with CMV (strain AD169 or VHL/E) at an MOI of 2 PFU/cell. At the indicated times postinfection, total cellular RNA was harvested and subjected to RNase protection analysis with OAS- and actin-specific probes. (B) CMV triggers an antiviral state in endothelial cells. HUVEC were stimulated as indicated (interferon, 100 U/ml). At 6 h poststimulation, the treatments were removed and the monolayers were challenged with approximately 100 PFU of VSV per well. Plaque formation was visualized by crystal violet staining at 48 h postinfection.