Fig. 4.

Expression of mesenchymal markers α-smooth muscle actin (SMA) and fibronectin and platelet-derived growth factor receptor (PDGFβ-R). The figure shows α-SMA and fibronectin were abundantly expressed in mesangial cells, whereas α-SMA expression in podocytes was low and was entirely absent in glomerular endothelial cells. Fibronectin was expressed in the glomerular endothelial cell as well and was weakly expressed in podocytes. Actin was used as a loading control. PDGFβ-R was expressed exclusively in mesangial cells.