Fig. 1.

Rat aortic smooth muscle cells (RASMCs) were transfected with p3xFlag/rAT₁R/myc for 18 h then serum-starved in 0.5% FBS containing vehicle (A) or ANG II (10⁻⁸ M) (B) for 3 h. Cells were then fixed, permeabilized, and stained for Flag (Alexa Fluor 555, red) and myc (Alexa Fluor 488, green). Note that red and green fluors are largely colocalized (as yellow fluorescence) in vehicle-treated cells (arrows), whereas ANG II treatment causes a separation of the two fluorescent moieties and increased accumulation of green fluorescence on the cell surface (arrowhead) and in the nucleus (n).