FIG. 1.
Scanned digital image of Coomassie-stained gels showing protein profiles from relevant steps of the purification of UL15 (A) and UL28 (B) proteins. (A) Lane 1, initial cell lysate; lane 2, supernatant after lysis; lane 3, supernatant from wash of insoluble material; lane 4, eluted protein after dialysis. (B) Lane 1, initial cell lysate; lane 2, supernatant after lysis; lane 3, Ni-nitrilotriacetic acid agarose beads after incubation with the insoluble, denatured protein fraction and extensive washing; lane 4, supernatant from wash of agarose beads; lane 5, eluted protein after dialysis. Molecular mass standards are indicated on the left in kilodaltons.