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. 2011 Jul 27;301(5):C1093–C1103. doi: 10.1152/ajpcell.00122.2011

Fig. 2.

Fig. 2.

Antithrombin (AT)-mediated MTP inhibition is seen in 184 A1N4 mammary epithelial cells. A: 184 A1N4 mammary epithelial cells were exposed to culture medium containing 0.5% human serum for 10 min. Then the cells were exposed to basal medium (lane 1) or a pH 6.0 buffer (lane 2) to induce MTP activation. Cell lysates were prepared and analyzed by immunoblot for MTP species by MTP mAb M24 or for antithrombin species using a human antithrombin polyclonal antibody (AT). B: to confirm that the 110-kDa antithrombin species is MTP-AT complex, the cell lysates (L) were immunoprecipitated by MTP mAb 21–9 (IP:MTP), and the captured MTP species were eluted by pH 2.4 glycine buffer. The samples from loading (L), unbound fraction (UB), and elute fraction (E) were analyzed by immunoblotting for AT species by an AT Ab (IB:AT).