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AT-mediated MTP inhibition is commonly seen in nontumorigenic mammary and prostate epithelial cells. MTSV-1.1B and MCF 10A mammary epithelial cells, RWPE-1 prostate epithelial cells, and HaCaT keratinocytes were exposed to culture medium containing human sera for 10 min. Then these cells were exposed to basal medium (lanes 1) or a pH 6.0 buffer (lanes 2) for 20 min to induce MTP activation. Cell lysates were prepared and analyzed by Western blotting for MTP and AT.
