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. 2011 Aug 3;301(5):C1150–C1160. doi: 10.1152/ajpcell.00203.2011

Fig. 2.

Fig. 2.

The expression of NKCC1 regulatory kinases WNK1, WNK2, WNK3 and WNK4 in glioma cell lines and human patient biopsy tissue of normal brain and gliomas, grades I–IV. Representative Western blots of WNK1 (A), WNK2 (C), WNK3 (E), and WNK4 (G) in grade IV glioma cell line whole cell lysates U251, U87, D54, and STTG1 as well as in more recent, locally derived glioma cell lines GBM50 and GBM62. HEK293 cell lysates serve as a positive control. Representative Western blots of WNK1 (B), WNK2 (D), WNK3 (F), and WNK4 (H) in lysates from normal human brain and glioma (grades I-IV) biopsies. (B = normal brain, I = glioma grade I, II = glioma grade II, III = glioma grade III, IV = glioma grade IV). Images are representative of at least 3 independent blots for each antibody. Actin and Na-K-ATPase serve as loading controls.