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NKCC1 and WNK3 expression in hyperosmotic-treated D54 human glioma cells. A: immunofluorescent images of D54 human glioma cells exposed to hyperosmotic conditions for 8 min, double labeled with antibodies targeting NKCC1 (red) and WNK3 (green). Nuclei are labeled with Hoechst (blue). B and C: digital magnifications of boxed cells in A. Scale bar 20 μm. N = 3.
