Fig. 7.

WNK3 knockdown abolishes regulatory volume increase (RVI) in human glioma cells. A: average normalized mean cell volumes (MCVs, n = 5,000–10,000 cells) of NS glioma cells undergoing RVI after hyperosmotic (15 mM NaCl) challenge in the presence or absence of 200 μM bumetanide. Experiments were performed three independent times. B: average normalized MCVs for WNK3-KD cells undergoing RVI after hyperosmotic challenge in the presence or absence of bumetanide. Experiments were performed three independent times. C: average NS and WNK3-KD cell normalized MCVs at 40 min post-hyperosmotic challenge. One-way ANOVA, P = 0.001, Tukey-Kramer post hoc test, **P < 0.01. D: image of Western blot showing WNK3 expression in NS and WNK3-KD cell lysates. Actin serves as a loading control. Blots are representative of N = 8 experiments from four different cell lysate preparations. E: normalized WNK3 expression in NS and WNK3-KD glioma cell lines. Unpaired t-test, P = 0.0009.