Fig. 3.

Reduced susceptibility of MLECs reexpressing Cav-2 to the time and concentration-dependent anti-proliferative effect of TGF-β. A and B: pBABE and Cav-2-pBABE MLECs were plated at 4 × 10³ cells per well of 24-well plate and incubated without or with TGF-β, followed by processing for MTT proliferation assay. OD was measured using a plate reader with a test wavelength of 570 nm and a reference wavelength of 750 nm (570–750 nm) to obtain a sample signal and expressed as means ± SD of replicate samples (n = 3). Values are from one representative out of a total four experiments. A: time-dependent effect of TGF-β (1 ng/ml) on MLEC proliferation up to 6 days. B: concentration-dependent effect of TGF-β (0.001–10 ng/ml) on MLECs proliferation determined after 5 days of treatment. C and D: pBABE and Cav-2-pBABE MLECs were plated at 10⁵ cells per 100-mm dish, cell numbers were determined at the indicated time points using Cell Countess. Values are from one representative out of a total four experiments. C: time-dependent effect of TGF-β (1 ng/ml) on MLEC proliferation up to 8 days. D: concentration-dependent effect of TGF-β (0.001–1 ng/ml) on MLECs proliferation determined after 5 days of treatment.