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. 2011 Aug 10;301(5):C1161–C1174. doi: 10.1152/ajpcell.00486.2010

Fig. 6.

Fig. 6.

Alk5 dependence of the anti-proliferative effect of TGF-β in MLECs. A: WT MLECs and Cav-2 KO MLECs expressing pBABE or Cav-2-pBABE were plated at 4 × 103 cells per well of 24-well plate and incubated without or with TGF-β (1 ng/ml and/or SB-5 (1 μM) for 6 days, followed by processing for MTT proliferation assay and measurement of OD as described in materials and methods. The data are expressed as means ± SD of replicate samples (n = 3). *P < 0.05 vs. respective TGF-β-treated sample; #P < 0.05 vs. respective control sample. B: WT MLECs and Cav-2 KO MLECs expressing pBABE or Cav-2-pBABE were plated at 3 × 105 per 150-mm dish and incubated without or with TGF-β (1 ng/ml) and/or SB-5 (1 μM) for 24 h, followed by lyses and immunoblotting with the indicated antibodies. Graphs, densitometric ratios of p27 (top) and P-Rb (bottom) to Hsp90 quantified using Image J and expressed as means ± SD (n = 3). *P < 0.05 vs. respective TGF-β-treated sample; #P < 0.05 vs. respective control sample.