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. 2011 Aug 10;301(5):C1161–C1174. doi: 10.1152/ajpcell.00486.2010

Fig. 9.

Fig. 9.

Detergent-free sucrose fractionation of TGF-β receptors and Smad2/3 in Cav-2-negative and -positive MLECs. WT and Cav-2 KO MLECs cells were lysed with sodium carbonate (pH 11) and sonicated, followed by a sucrose floatation gradient as described in materials and methods. Equal amounts of protein were loaded for pooled and concentrated top 3 light (L) fractions, bottom pooled 3 heavy (H) fractions and compared with total (T) cell lysates. A: immunoblotting with indicated antibodies and staining with Coomassie blue (CB) (compressed by 3-fold in the vertical dimension). B–H: relative (%) distribution of densitometric signal values expressed as means ± SD (n = 3) for given proteins normalized against CB between L and H.