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Aldosterone stimulates vacuolar H+-ATPase activity in freshly isolated human OMCD intercalated cells. OMCDs were incubated with 10 nM aldosterone for 20 min or were left untreated (control). The summary of Na+-independent pHi alkalinization rates in single intercalated cells in freshly isolated human OMCDs in the absence and presence of aldosterone (10 nM, 20 min preincubation) is shown. Values are presented as means ± SE. ***P < 0.001.
