Fig. 4.
Aldosterone acts via intracellular Ca2+ and protein kinase C. Chelation of intracellular Ca2+ with BAPTA-AM (100 μM) prevented the aldosterone (10 nM) effect. Similarly, inhibition of protein kinase C activity with chelerythrine (Chel, 1 μM) completely blocked the stimulation by aldosterone. On the other hand, stimulation of PKC activity with DOG (1 μM) mimicked the effect of aldosterone. The values of control and aldosterone-treated OMCDs are shown again for comparison. Values are presented as means ± SE. ***P < 0.001 significantly different from control, ###P < 0.001 significantly different from aldosterone alone.