Fig. 6.

The stimulatory effect of aldosterone is mediated via ERK1/2 kinases. OMCDs were incubated with the ERK1/2 inhibitor PD098059 (20 μM) in the absence and presence of aldosterone (10 nM). PD098059 abolished the stimulatory effect of aldosterone. Coincubation of OMCDs with the PKC activator DOG (1 μM) and the ERK1/2 inhibitor PD098059 showed a small stimulation which was less pronounced than with DOG alone (see also Fig. 4). The values of control and aldosterone-treated OMCDs are shown again for comparison. Values are presented as means ± SE. ***P < 0.001 significantly different from control, ###P < 0.001 significantly different from aldosterone alone.