Fig. 3.

KCNQ4 protein expression in neural retina and RPE. A: anti-KCNQ4 antibody immunolabeled a ∼77 kDa band in Chinese hamster ovary (CHO) cells transfected with KCNQ4_v1 (lane 2) or KCNQ4_v2 (lane 3), but not in untransfected cells (lane 1). B: in retina crude membrane protein, anti-KCNQ4 antibody labeled ∼77 and ∼154 kDa bands (left), which were diminished after the antibody was preabsorbed with antigenic peptide (right). C: KCNQ4 was not detected in blots of RPE plasma membrane. Results are representative of more than 3 separate experiments.