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Intracellular localization of wt and AM5 mutant MPs in insect cells and cowpea protoplasts. (A through C) Immunofluorescent detection of MP in insect cells expressing AM5MP (A and B) and wtMP (C); (D and E) cowpea protoplasts transiently expressing AM5MP-YFP (D) and wtMP-YFP (E). Arrows indicate tubules formed at the cell surface. Bar, 5 μm.
