FIG. 4.

Nucleic acid binding of CPMV MP, evaluated by gel shift assay. The protein-RNA complex and the free RNA were separated by electrophoresis in a 1% agarose gel. Fifty nanograms of ³²P-labeled ssRNA corresponding to the C-terminal 707 nt of CPMV RNA2 was used in all experiments. For reasons yet unknown, two RNA molecules were produced during in vitro transcription. (A) Binding of different amounts of MP to ssRNA; (B) binding of CPMV MP (500 ng) to ssRNA under increasing NaCl concentrations; (C) various amounts of AM5MP incubated with 50 ng of ³²P-labeled ssRNA. The wtMP was used as a control.