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Surface labeling of GFP-gB and the truncated forms in transfected cells. Cos-7 cells were fixed under nonpermeabilizing conditions 24 h after transfection with pGFP-gB or each of the truncated forms and were labeled with an anti-gB antibody and a Texas red-labeled secondary antibody. Fluorescence was visualized with a Zeiss Axiophot conventional microscope.
