FIG. 7.

Effect of mutation of the LL motif on internalization. After transfection, LL871AA-expressing cells were incubated with an anti-GFP monoclonal antibody for 1 h and then returned to 37°C for different times. After methanol fixation and labeling with an anti-TGN 46 antibody, cells were incubated with Cy3- and Cy5-labeled secondary antibodies. Panels A to D, E to H, I to L, and M to P correspond to 0-, 15-, 30-, and 60-min incubation times, respectively, at 37°C. (A, E, I, and M) GFP fluorescence; (B, F, J, and N) anti-GFP immunofluorescence; (C, G, K, and O) TGN 46 localization; (D, H, L, and P) merged images.