FIG. 9.

Complementation of K082 virus by GFP-gB or the mutated Y889A, LL871AA, Δ857, or Δ849 protein. Vero cells were transfected with plasmids expressing GFP-gB or the mutated proteins and were infected 24 h later with K082 at a multiplicity of infection of 1. Extracellular virions were washed off at 1 h postinfection by treatment with an acid-glycine-saline solution, followed by three washes with PBS. Infected cell culture supernatants were harvested 24 h after infection and titrated on D6 and Vero cells. Titers shown are the averages from three independent experiments.