TABLE 1.
Oligonucleotides used to generate HSV-1 gB mutations
| Constructiona | Position of truncation or description of mutation | Oligonucleotide (5′ → 3′) |
|---|---|---|
| Truncations | ||
| Δ896 | From aa 896 to stop codon | ATCGGATCCTCATTTGTTGGGAACTTGGGT |
| Δ889 | From aa 889 to stop codon | TATGGATCCTCAGTTGGTGTTGCGGCGCTT |
| Δ871 | From aa 871 to stop codon | TATGGATCCTCACGCGCTCGTGCCCTTCTT |
| Δ857 | From aa 857 to stop codon | TATGGATCCTCACATGGCCGACACCAGGGC |
| Δ849 | From aa 849 to stop codon | TATGGATCCTCACCGTATCATCTCCCTGGC |
| Point mutations | ||
| Y889A | Y889 to A | CGCCGCAACACCAACGCCACCCAAGTTCCCAAC (5′) |
| GTTGGGAACTTGGGTGGCGTTGGTGTTGCG (3′) | ||
| LL871AA | L871 and L872 to AA | GGCACGAGCGCGGCGGCCAGCGCCAAGGTC (5′) |
| GACCTTGGCGCTGGCCGCCGCGCTCGTGCC (3′) |
a
Single-letter amino acid abbreviations are used. Numbers represent amino acid positions within HSV-1 gB, including its signal peptide.