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Western immunoblot showing the distribution of RhoA in membrane (M) and cytosol (C) of healthy human erythrocytes (A). Membrane and cytosolic fractions of healthy human erythrocytes were incubated with a RhoA polyclonal antibody generated against an internal region of RhoA. Twenty-five micrograms of total protein were loaded per lane, and values are reported as means ± SE, n = 3 experiments (B).
